Research Faculty

Mini Jose Deepak

Ramalingaswami Fellow

Phone : +91 80 2293 2518
E-Mail : mini[at]

Research Areas

Differentiation of neuronal processes into subtypes

Research Details

Differentiation of neuronal processes into subtypes namely, axons and dendrites remain to be a highly intriguing but critical mechanism for survival during neuronal development.

Interestingly, though different approaches have been adopted to intercept the molecular mechanism behind, a clear model on this critical transition during development remains unclear. In my project, I try to address this interesting question by adopting a multidisciplinary approach combining molecular biology and single molecule based superresolution microscopy to study the role of lipid metabolism in neuronal differentiation using mouse hippocampal neurons as the model system.


Mangalwedhekar R., Singh N., Thakur C.S., Seelamantula C.S., Jose M., Nair D., (2022), Achieving nanoscale precision using Neuromorphic localization microscopy,, Nature Nanotechnology, (Accepted).

Rajeev P., Singh N., Kechkar A., Butler C., Ramanan N., Sibarita J.B., Jose M., Nair D. , (2022), Nanoscale Regulation of Ca2+ dependent phase transitions and real time dynamics of SAP97/hDLG, Nature Comm., 2022. 13(1): 4236.

Jose M.*, Sivanand A., Channakeshava C., (2021), Cholesterol is a critical determinant for hippocampal neuronal polarity, Frontiers in Mol. Neuroscience, (in Press).

Kedia S., Mandal K., Netrakanti P.R., Jose M., Sisodia S.S., Nair D., (2021), Nanoscale organization of Nicastrin, the Substrate Receptor of the γ-Secretase Complex, as Independent Molecular Domains, Molecular Brain, (in Press).

Tanwar M., Kateriya S., Nair D., Jose M*, (2021), Optogenetic modulation of real-time nanoscale dynamics of HCN channels using photoactivated adenylyl cyclases, RSC Chemical Biology, 2(3), 863-875.

Kedia S., Ramakrishna P., Netrakanti P.R., Sisodia SS, Jose M., Kumar S, Mahadevan A, Ramanan N. Nadkarni S., Nair D., (2021), Alteration in synaptic nanoscale organization dictates amyloidogenic processing in Alzheimer’s disease, IScience, 24(1), 101924.

Kedia, S., Ramakrishna, P., Netrakanti, P. R., Jose, M., Sibarita, J. B., Nadkarni, S., & Nair, D., (2020), Real-time nanoscale organization of amyloid precursor protein, Nanoscale, 12(15), 8200-8215.

Venkatesan, S., Subramaniam, S., Rajeev, P., Chopra, Y., Jose, M., & Nair, D, (2020), Differential scaling of synaptic molecules within functional zones of an excitatory synapse during homeostatic plasticity, ENeuro, 7(2).

Nanguneri, S., Pramod, R. T., Efimova, N., Das, D.,Jose, M., Svitkina, T., & Nair, D., (2019), Characterization of nanoscale organization of f-actin in morphologically distinct dendritic spines in vitro using supervised learning, Eneuro, 6(4).

Venkatachalapathy, M., Belapurkar, V., Jose, M., Gautier, A., & Nair, D, (2019), Live cell super resolution imaging by radial fluctuations using fluorogen binding tags, Nanoscale, 11(8), 3626-3632.

Sartorel E, Ünlü C, Jose M, Massoni-Laporte A, Meca J, Sibarita JB, McCusker D, (2018), Phosphatidylserine and GTPase activation control Cdc42 nanoclustering to counter dissipative diffusion, Molecular Biology of the Cell, 29(11), 1299-1310

Jose M., Tollis S., Nair D.K., Mitteau R, Velours C, Massoni-Laporte A, Rouyou A, Sibarita J.B., McCusker D, (2015), A quantitative imaging-based screen reveals the exocyst as a network hub connecting endocytosis and exocytosis, Mol Biol Cell, 26(13):2519-34.

Jose M., Tollis S., Nair D.K., Sibarita J.B., McCusker D, (2013), Robust polarity establishment occurs via an endocytosis-based cortical corralling mechanism, J Cell Biol, 200(4): 407-18

Jose M.*, Nair D. K., Altrock W. D., Dresbach T., Gundelfinger E. D., and Zuschratter W, (2008), Investigating interactions mediated by the presynaptic protein bassoon in living cells by Foerster’s resonance energy transfer and fluorescence lifetime imaging microscopy, Biophys J, 94(4):, 1483-1496

Jose M.*, Nair D. K., Reissner C., Hartig R., and Zuschratter W, (2007), Photophysics of Clomeleon by FLIM: Discriminating Excited State Reactions along Neuronal Development, Biophys J, 92(6):, 2237-2254

Nair D. K.°, Jose M.°, Kuner T., Zuschratter W., and Hartig R, (2006), FRET-FLIM at nanometer spectral resolution from living cells, Optics Express, 14(25):, 12217-12229

Rajesh R. J., Jose M., and Bisht P. B, (2002), Diffraction efficiency of transient grating and relaxation profiles of some laser dyes, Ind. J. Physics, 76B:, 737-742